The LH Quantitative Test is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay system utilizes a goat anti-α-LH antibody for solid phase (microtiter wells) immobilization and a mouse monoclonal anti-β-LH antibody in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react simultaneously with the antibodies, resulting in LH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After a 45 minute incubation at room temperature, the wells are washed with water to remove unbound-labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution, and the color is changed to yellow and measured spectrophotometrically at 450nm. The concentration of LH is directly proportional to the color intensity of the test sample.