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Triglyceride

Catalog #: OT932-780


METHOD

Enzymatic/GPO Trinder, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Linearity: 1000 mg/dL
Expected Values: 44-148 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 12 x 65 ml
Test/Kit: 4333
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
  • Description
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Intended Use
For the in vitro quantitative determination of Triglycerides in serum or plasma. For in vitro diagnostic use only.
Principle
Serum triglycerides are hydrolyzed to glycerol and free fatty acids by lipase. In the presence of ATP and glycerol kinase (GK), the glycerol is converted to glycerol-1-phosphate. The glycerol-1-phosphate is then oxidized by glycerol phosphate oxidase (GPO) to yield hydrogen peroxide. The condensation of hydrogen peroxide with 4-chlorophenol and 4-aminophenazone (4-AA) in the presence of peroxidase (POD) produces a red colored quinoneimine dye which absorbs at, or near 505nm. The intensity of the colored complex formed is directly proportional to the triglycerides concentration of the sample.