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Creatine Kinase

Catalog #: 12-C7522-100


METHOD

CK-NAC, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Linearity: 1-1,200 U/L
Expected Values: Males: 38 - 174 U/L, Females: 26 - 140 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 2 x 40 ml/2 x 10 ml
Test/Kit: 400
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
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Intended Use
For the quantitative determination of creatine kinase activity in serum, using the Mindray BS-200 analyzer.
Principle
The kinetic procedure presented is a modification of Szasz of the Rosalki technique, which optimizes the reaction by reactivation of CK activity with N-actyl-L-cysteine (NAC).

CK specifically catalyzes the transphosphorylation of ADP to ATP. Through a series of coupled enzymatic reactions, NADPH is produced at a rate directly proportional to the CK activity. The method determines the NADPH absorbance increase per min at 340 nm.