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Acid Phosphatase

Catalog #: 15-A7503-100


METHOD

α-naphthylphosphate, Kinetic

TECHNICAL INFORMATION

Format: Dry Powder
Wavelength: 405 nm
Linearity: 40 U/L
Expected Values: Total Acid Phosphatase: 2.5-11.7 U/L, Prostatic Acid Phosphatase: 0.2-3.5 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: 24 hrs at 15-30°C,14 days at 2-8°C
Configuration: 10 x 10 mL
Test/Kit: 714
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
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Intended Use
For the quantitative determination of acid phosphatase in serum using the Tokyo Boeki Medisys Inc. Biolis 24i analyzer.
Principle
α-naphthylphosphate is hydrolyzed by serum acid phosphatase to α-napthol and inorganic phosphate. The rate of hydrolysis is proportional to the enzyme acitivity present. The α-naphthol produced is coupled with Fast Red TR to produce a colored complex which absorbs light at 405 nm. The reaction can be quantitated photometrically because the coupling reaction is instantaneous. L-Tartrate inhibits prostatic acid phosphatase but does not interfere with the reaction mechanism. Therefore, if testing is performed in the presence and in the absence of L-Tartrate, the difference between the results of the two assays is the level of prostatic acid phosphatase in the serum.