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Amylase

Catalog #: 15-A7564-76


METHOD

CNPG3, Kinetic

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 405 nm
Linearity: 1-2,000 U/L
Expected Values: 25-125 U/L
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 2 x 38 mL
Test/Kit: 506
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
  • Description
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Intended Use
For the quantitative kinetic determination of α-Amylase activity in human serum using the Tokyo Boeki Medisys Inc. Biolis 24i analyzer.
Principle
α-Amylase hydrolyzes the 2-chloro-p-nitrophenyl-α-D-maltotrioside (CNPG3) to release 2-chloro-nitrophenol and form 2-chloro-p-nitrophenyl-α-D-maltoside (CNPG2), maltotriose (G3) and glucose (G). The rate of increase in absorbance is measured at 405 nm and is proportional to the α-amylase activity in the sample.