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CE Marked Health Canada

Cholesterol

Catalog #: 15-C7510-152


METHOD

Enzymatic, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 500 nm
Linearity: 500 mg/dL
Expected Values: Desirable Cholesterol: <200 mg/dL, Borderline-High Cholesterol: 200-239 mg/dL, High Cholesterol: >240 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 4 x 38 mL
Test/Kit: 760
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
  • Description
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Intended Use
For the quantitative determination of Total Cholesterol in serum using the Tokyo Boeki Medisys Inc. Biolis 24i analyzer.
Principle
Cholesterol esters in the sample are hydrolyzed by cholesterol esterase to release free cholesterol. The free cholesterol is oxidized by cholesterol oxidase to release hydrogen peroxide. Phenol + 4-AAP + hydrogen peroxide, in the presence of peroxidase produces a quinoneimine dye that is measured at 500nm. The absorbance at 500nm is proportional to the concentration of cholesterol in the sample.