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Glucose Hexokinase

Catalog #: 15-G7517-152


METHOD

Hexokinase/G6PD, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 340 nm
Linearity: 0.7-600 mg/dL
Expected Values: 70-105 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 4 x 38 mL
Test/Kit: 760
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
  • Description
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Intended Use
For the in vitro quantitative measurement of glucose in serum.
Principle
Glucose is phosphorylated by hexokinase (HK) in the presence of adenosine triphosphate (ATP) and magnesium to form glucose-6-phosphate (G-6-P) and adenosine diphosphate (ADP). G-6-P is then oxidized by glucose-6-phosphate dehydrogenase (G-6-PDH) in the presence of nicotinamide adenine dinucleotide (NAD) producing 6-phosphogluconate and NADH.
The formation of NADH causes increase in absorbance at 340 nm which is directly proportional to the concentration of glucose in the sample.