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Triglyceride

Catalog #: 15-T7532-152


METHOD

Enzymatic/GPO Trinder, Endpoint

TECHNICAL INFORMATION

Format: Liquid
Wavelength: 500 nm
Linearity: 1-1000 mg/dL
Expected Values: 44-148 mg/dL
Storage Temperature: 2-8°C
Reconstituted Stability: Ready to use liquid
Configuration: 4 x 38 mL
Test/Kit: 760
Disclaimer: Actual test/kit numbers may be lower due to instrument limitation.
  • Description
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Intended Use
For the in vitro quantitative determination of Triglycerides in serum or plasma using the Tokyo Boeki Medisys Inc. Biolis 24i analyzer.
Principle
Serum triglycerides are hydrolyzed to glycerol and free fatty acids by lipase. In the presence of ATP and glycerol kinase (GK), the glycerol is converted to glycerol-1-phosphate. The glycerol-1-phosphate is then oxidized by glycerol phosphate oxidase (GPO) to yield hydrogen peroxide. The condensation of hydrogen peroxide with 4-chlorophenol and 4-aminophenazone (4-AA) in the presence of peroxidase (POD) produces a red colored quinonimine dye which absorbs at, or near 500nm. The intensity of the colored complex formed is directly proportional to the triglycerides concentration of the sample.