Overview
Advancements in Neuronal Cell Cultures
Neuronal cell cultures are essential in neuroscience research. They help researchers understand neuron function, study diseases, develop drugs, and assess neurotoxicity. Keeping neurons alive and functioning as they do in the brain has always been a challenge. Gibco B-27 Plus Neuronal Culture System by Thermo Fisher Scientific is a game-changer in this field.
Importance of Long-term Neuron Viability
Neurons are sensitive to their environment. When taken out of the brain, they require specific conditions to survive. Traditional culture media often fall short, leading to a decline in neuron health. Usually, primary rodent neurons survive only around 14 days in vitro with existing media systems. Maintaining cell health and functionality beyond this period is critical for in-depth studies.
Enhancements in Culture Media
The B-27 Plus Medium offers significant improvements in neuron viability and function over longer periods. This new system is optimized for primary rodent neurons and human stem cell-derived neurons, providing an ideal environment for both low and high-density cultures.
Key Improvements of B-27 Plus:
- Optimized formulation
- Enhanced manufacturing processes
- Rigorous quality control
Independent Evaluation by Axion BioSystems
Using the Maestro Pro microelectrode array (MEA) platform, Axion BioSystems verified the B-27 Plus system. Neurons were assessed at day 7, 14, and 21, focusing on neurite outgrowth, viability, and functionality. Quantitative morphometric analysis using the IncuCyte Zoom Live-Cell Analysis System provided clear insights.
Findings from Evaluation:
- Increased neuronal cell numbers
- Enhanced neurite outgrowth
- Improved synaptic maturity
Neuronal Survival Rates
Survival rates increased by over 50% compared to older media. This ensures longer and more reliable experiments. For example, primary rat cortex neurons cultured with B-27 Plus showed higher survival rates when immunostained for MAP2 at days 7, 14, and 21.
Extended Research Periods
Before the B-27 Plus, studies using mouse primary hippocampal neurons would last a maximum of 14 days. With the new system, these neurons can be cultured for up to 28 days with sustained viability and functionality.
Neurite Outgrowth Enhancement
Neurite outgrowth is critical for neuron networking. The B-27 Plus system accelerates this process, especially in short-term cultures. The IncuCyte NeuroTrack analysis tool quantified neurite length, demonstrating significant improvement compared to the classic B-27 system.
Synaptic Connections and Network Activity
Mature neuron networks exhibit synchronized activity due to extensive synaptic connections. The B-27 Plus system facilitates stronger network development. This was shown by increased neural activity and better network synchronization.
Summary of Benefits
| Feature | Classic B-27 | B-27 Plus |
|---|---|---|
| Neuronal survival | Medium | High |
| Neurite outgrowth | Moderate | Accelerated |
| Synaptic maturity | Basic | Enhanced |
| Viability period | ~14 days | ~28 days |
| Network activity | Standard | Superior |
Versatile Application
The B-27 Plus system is versatile, benefitting various areas:
- Primary rat neurons
- Human stem cell-derived neurons
- Neurodegenerative disease research
- Drug development
Optimized for Neuroscience Researchers
The B-27 Plus system is tailored for those doing advanced neuroscience research. It provides a stable, reliable platform for long-term studies of neuron development and function. Researchers can now maintain neuronal cultures with better survival rates and more mature networks over extended periods.
Conclusion
The B-27 Plus Neuronal Culture System represents a significant advancement in the field. With enhanced neuron viability, accelerated neurite outgrowth, and improved network activity, it supports more extended and in-depth research. This new system is a valuable tool for anyone looking to conduct cutting-edge neuroscience investigations.
